LabNet

Introduction

LabNet was inaugurated at a meeting in Noumea, New Caledonia, in April 2000.

It is a three-level network of laboratories (L1, L2 and L3), where ‘level’ refers not to the capacity of the lab but to its role in the network.

L1 labs are the labs of the PPHSN member countries and territories. This level is the closest to patients and clinicians facing an outbreak alert. L1 labs collect samples from patients in suspect cases and when possible use screening tests. They should have ready access to L2 or L3 lab confirmation services for selected diseases.

L2 regional labs perform the first level of confirmation testing for some L1 labs. The techniques they employ require more training and equipment than those used at L1 labs. They are further supported by L3 labs. L2 regional labs also serve as L1 labs for those countries or territories in which they are located.

L3 labs are reference laboratories. They are usually internationally recognised labs (e.g. WHO-associated centres) located in the Pacific rim countries (mainly Australia, New Zealand and USA). In addition to the L2 disease-specific role, they usually perform more sophisticated, less urgently needed tests, providing important epidemiological information for the region or in the context of the worldwide surveillance of major diseases (such as dengue virus genotyping, influenza subtyping or Leptospira serogrouping).

Highlights

WD # 4 Referral of biological samples in the PacificC PPHSN LABNET Reference laboratory

PPHSN LabNet reference Laboratories

Recent articles published in Inform’ACTION:
Developing sustainable influenza surveillance networks in the Pacific

Influenza diagnosis – from rapid testing to Polymerase Chain Reaction (PCR) in the Pacific

Sensitivity and specificity of rapid diagnostic tests and immunofluorescence assay for influenza

Assessment of NS1 antigen detection tests during DEN-4 epidemic in French Polynesia

LabNet gives recommendations and support for shipping and testing of specimens and has a dedicated email communication list (PacNet-Lab).

A Technical Working Body (TWB), was created for the facilitation and coordination of LabNet development. This working group has been revived with new members and revised terms of references (see the outcomes of the teleconference organised in April 2011) .

Contact:
For any further information please contact the Technical Working Body

Activities & Projects

Increasing influenza surveillance in the Pacific Island Region
An influenza surveillance programme modelled on one already operating in New Caledonia is being implemented in the Pacific Island region. This project, which started in 2006, has now been fully implemented in 14 sites in 13 Pacific Island countries and territories.

Objective
he programme aims at describing influenza virus circulation in Pacific Island countries and territories (PICTs).

PPHSN partnership
The programme is funded by the Centers for Disease Control and Prevention (CDC), coordinated and implemented by the Secretariat of the Pacific Community (SPC) and the Pasteur Institute of New Caledonia (IPNC) in partnership with WHO Collaborating Centre for Influenza (WHO-CC), and other laboratory and training institutions in the region, such as the Pacific Paramedical Training Centre (PPTC) and the Institute for Environmental Science and Research (ESR) in New Zealand.

Proceedings
It involves obtaining basic epidemiological data and respiratory specimens from both hospital and regional health care centre patients, along with laboratory testing of the samples.

Immunofluorescent staining of these clinical samples is performed in the PICTs laboratories for the detection of influenza. Confirmatory testing is performed at the Pasteur Institute of New Caledonia (IPNC) on ethanol-fixed nasal specimens using Real-Time PCR, with additional confirmation and typings performed at WHO-CC.

Surveys

  • Leptospirosis survey in the Pacific: Progress report, August 2004 (PDF, 35 KB)
  • Multi-centre Survey on Incidence and Public Health Impact of leptospirosis in the Pacific (PDF, 62 KB)

Evaluations of Reagents
In 2004, the Pasteur Institute of New Caledonia and the State Hospital in Yap (FSM) carried out a study to evaluate a new rapid test for dengue serology. The results of the evaluation have been published in WHO Dengue Bulletin – Vol 29, 2005

In August 2002, the Pasteur Institute of New Caledonia performed evaluations of existing rapid diagnostic kits for leptospirosis and dengue. The results are detailed in the reports below:

  • Evaluation of reagents for the serological diagnosis of Dengue – New Caledonia Pasteur Institute (PDF file, 457 KB)
  • Evaluation of reagents for the serological diagnosis of Leptospirosis – New Caledonia Pasteur Institute (PDF file, 547 KB)

Laboratory Assessments

  • Report of a consultation: Pacific Public Health Laboratory Review in Guam, Saipan, Pohnpei, Majuro and Honolulu, 9–13 February 2004 (PDF, 52 KB) or (Word, 139 KB)
  • LabNet: Laboratory technical re-assessment – Level 1 laboratories – 2003 (PDF, 46 KB) or (Word, 156 KB)

Photos